lentiviral vector backbone Search Results


third generation lentiviral transfer vector backbone pelns xbai kozak β2m gs linker mr1  (ATCC)
96
ATCC third generation lentiviral transfer vector backbone pelns xbai kozak β2m gs linker mr1
Third Generation Lentiviral Transfer Vector Backbone Pelns Xbai Kozak β2m Gs Linker Mr1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/MR1/pmc12461623__mmc7-484-97-94
Average 96 stars, based on 1 article reviews
third generation lentiviral transfer vector backbone pelns xbai kozak β2m gs linker mr1 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
backbone lentiviral vector  (New England Biolabs)
97
New England Biolabs backbone lentiviral vector
Backbone Lentiviral Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/SmaI/pmc10662150-364-1-9
Average 97 stars, based on 1 article reviews
backbone lentiviral vector - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier
lentiviral plasmid backbone  (VectorBuilder GmbH)
90
VectorBuilder GmbH lentiviral plasmid backbone
Lentiviral Plasmid Backbone, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/a+lentiviral+plasmid+backbone/10__1158_slash_2326___6066__cir___21___0062-68-18-21
Average 90 stars, based on 1 article reviews
lentiviral plasmid backbone - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
lentiviral vector backbone  (Addgene inc)
93
Addgene inc lentiviral vector backbone
Lentiviral Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/Lentiviral-TOP-dGFP-reporter+(Plasmid+%2314715)/10__1074_slash_jbc__m113__453985-56-9-12
Average 93 stars, based on 1 article reviews
lentiviral vector backbone - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
plko 1 lentiviral vector backbone  (Addgene inc)
96
Addgene inc plko 1 lentiviral vector backbone
Plko 1 Lentiviral Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/pLKO%2E1+-+TRC+cloning+vector+(Plasmid+%2310878)/pmc05330761-238-12-16
Average 96 stars, based on 1 article reviews
plko 1 lentiviral vector backbone - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
lentiviral backbone vectors  (Addgene inc)
93
Addgene inc lentiviral backbone vectors
( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the <t>lentiviral</t> backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.
Lentiviral Backbone Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/Dual-sgRNA_hU6-mU6+(Plasmid+%23154194)/pmc12594195-206-0-22
Average 93 stars, based on 1 article reviews
lentiviral backbone vectors - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
lentiviral pcdh cmv mcs vector backbone  (Addgene inc)
94
Addgene inc lentiviral pcdh cmv mcs vector backbone
( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the <t>lentiviral</t> backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.
Lentiviral Pcdh Cmv Mcs Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/pCDH-CMV+(Plasmid+%2372265)/pmc09110333-412-8-12
Average 94 stars, based on 1 article reviews
lentiviral pcdh cmv mcs vector backbone - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
library transfer vector plas  (Addgene inc)
95
Addgene inc library transfer vector plas
( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the <t>lentiviral</t> backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.
Library Transfer Vector Plas, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/lenti+sgRNA(MS2)_zeo+backbone+(Plasmid+%2361427)/bio_rxiv__262121-75-16-27
Average 95 stars, based on 1 article reviews
library transfer vector plas - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
pcmv6 ac gfp lentiviral backbone expressing turbogfp  (OriGene)
96
OriGene pcmv6 ac gfp lentiviral backbone expressing turbogfp
( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the <t>lentiviral</t> backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.
Pcmv6 Ac Gfp Lentiviral Backbone Expressing Turbogfp, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/pCMV6-AC-GFP+Mammalian+Expression+Vector/pm30012602-249-3-8
Average 96 stars, based on 1 article reviews
pcmv6 ac gfp lentiviral backbone expressing turbogfp - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
lentiviral backbone vector pklv2 u6grna5 bbsi pgkpuro2amcherry w  (Addgene inc)
93
Addgene inc lentiviral backbone vector pklv2 u6grna5 bbsi pgkpuro2amcherry w
( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the <t>lentiviral</t> backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.
Lentiviral Backbone Vector Pklv2 U6grna5 Bbsi Pgkpuro2amcherry W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+backbone/pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W+(Plasmid+%2367977)/bio_rxiv__859652-141-1-5
Average 93 stars, based on 1 article reviews
lentiviral backbone vector pklv2 u6grna5 bbsi pgkpuro2amcherry w - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the lentiviral backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.

Journal: Science Advances

Article Title: Oligo-CALL: A next-generation barcoding platform for studying resistance to targeted therapy

doi: 10.1126/sciadv.adw9990

Figure Lengend Snippet: ( A ) Inducible gRNA barcode design: A spacer-blocking hairpin structure is engineered to include a 15-base semirandom genetic barcode within the hairpin loop. In addition, an 8A8G poly(A) tail is appended at the 3′ end of the barcoding gRNA. ( B ) Oligo-CALL system constructs: (i) Dual gRNA barcoding construct: two distinct barcoding gRNA, each driven by human or mouse U6 promoters, separately, with Thy1.1 as a constitutive selection marker. (ii) The green fluorescent protein (GFP) reporter construct: contains three tandem repeats of the barcoding gRNA target site upstream of a miniCMV (minimal cytomegalovirus) promoter controlling GFP expression; blue fluorescent protein (BFP) serves as a selection marker. (iii) CRISPRa construct: constitutively expresses dCas9-VPR [deactivated Cas9 fused to a transcriptional activator VPR (VP64, p65, and Rta)] and the red fluorescent protein (RFP; Cherry variant). All constructs use the lentiviral backbone to facilitate efficient cell transduction. hU6, human U6; mU6, mouse U6; EF1α, elongation factor-1 alpha promoter. ( C ) GFP activation with Oligo-CALL system: In the default state, a self-folding hairpin within the gRNA spacer prevents CRISPRa from activating GFP expression. Introduction of BC-ASOs disrupts this hairpin, exposing the gRNA spacer and enabling robust GFP expression. Representative fluorescence images show strong GFP induction following BC-ASO transfection, in contrast to minimal activation in cells receiving no ASO or mismatched ASOs (mis ASOs). ( D to F ) Flow cytometry analyses of GFP activation in Oligo-CALL–labeled cells: H358 cells bearing BC1 barcodes were transfected with ASOs against BC1. Flow cytometry shows the percentage of GFP + cells (D and E) and the mean fluorescent intensity (F) across the overall population (48 hours post-ASO transfection; n = 2 biological replicates). MFI, mean fluorescence intensity. Cont., control.

Article Snippet: Lentiviral backbone vectors—Dual-sgRNA (plasmid #154194), CaTCH_empty (plasmid #157746), and dCas9-VPR_P2A_mCherry (plasmid #154193), developed initially in A. Obenauf’s laboratory ( )—were obtained from Addgene.

Techniques: Blocking Assay, Construct, Selection, Marker, Expressing, Variant Assay, Transduction, Activation Assay, Fluorescence, Transfection, Flow Cytometry, Labeling, Control